Synthesis of Oligonucleotide Conjugates via Aqueous Diels‐Alder Cycloaddition
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چکیده
منابع مشابه
Drug targeting: synthesis and endocytosis of oligonucleotide-neoglycoprotein conjugates.
Inhibition of gene expression by antisense oligonucleotides is limited by their low ability to enter cells. Knowing that sugar binding receptors, also called membrane lectins, efficiently internalize neoglycoproteins bearing the relevant sugar, 6-phosphomannose, for instance, oligonucleotides--substituted on their 5'-end with either a fluorescent probe or a radioactive label on the one hand, an...
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The preparation and circular dichroic (CD) studies of self-complimentary 8-mer DNA sequences with a porphyrin at the 3' end are presented. Electronic interaction between the two porphyrins (the interchromophoric distance is in the range of 28-40 A), attached to both ends of the double-stranded helix, gives rise to a long-range exciton-coupled CD in the visible region (400-450 nm). The porphyrin...
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The underlying idea of oligonucleotide conjugates is to provide oligonucleotide with some novel property [...].
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The conjugation of oligonucleotide phosphorothioates with antennapedia peptide was studied in detail to allow efficient preparation of the conjugates on up to 15 mumol scale. Under optimized conditions, the use of oligonucleotides and the peptide in an equimolecular ratio gave the desired conjugates in more than 60% isolated yield.
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ژورنال
عنوان ژورنال: Current Protocols in Nucleic Acid Chemistry
سال: 2003
ISSN: 1934-9270,1934-9289
DOI: 10.1002/0471142700.nc0418s14